10 Ways to Induce Autophagy: When Does It Start?

Article based on science

What Is Autophagy? | Definition | Nobel Prize | Fasting | When Does It Start? | And Stop? | How to Induce Autophagy | 4 Methods | 6 Foods | 7 Benefits of Autophagy | Conclusion | FAQ | Studies

How can we induce autophagy? And when does it start? Learn about methods and foods that promote autophagy backed by science.

Intermittent Fasting 16/8 for Women Book

Initially, there was a balance in humankind between feasting and fasting. Accordingly, almost every religion includes a fasting custom.

However, we experience an endless summer in terms of nutrition today. Thus, our endless feasting causes modern diseases characterized by too much growth.

Autophagy is a process on the cell level induced by fasting, which we hardly use anymore.

Nevertheless, it’s precisely autophagy that can recycle cell waste and prevent those diseases ranging from obesity to cancer.

What Is Autophagy Fasting?

The word autophagy is derived from the Greek words auto (self) and phagein (to eat). Therefore it means “to eat oneself.”

Autophagy was first described in 1962 when researchers observed an increase in the number of lysosomes. These specific organelles broke down cell components in the liver cells of rats after the injection of glucagon.

In short, autophagy is a type of cell cleanse mechanism. In this process, the body identifies old and dysfunctional cell components and marks them for elimination.

Accordingly, autophagy can counteract the accumulation of old and broken cell parts responsible for the significant effects of aging and age-related diseases. These include, among others:

  • Alzheimer
  • Atherosclerosis (heart attack and stroke)
  • Fatty liver disease
  • Obesity
  • Cancer
  • Parkinson
  • Polycystic kidney disease
  • Polycystic ovary syndrome (PCOS)
  • Type 2 diabetes

Autophagy Definition

Autophagy is the mechanism in our body that sorts out or recycles disused organelles, proteins, and cell membranes.

Moreover, autophagy starts when there is a lack of energy to maintain these decayed cell parts. With this in mind, it’s a regulated and structured process of degradation and recycling of cellular waste.

Damaged subcellular parts and redundant proteins are marked for destruction and then sent to lysosomes.

Autophagy is similar to what is perhaps better known as programmed cell death or apoptosis.

Apoptosis programs cells to die after a certain number of divisions. Although this sounds a bit harsh, this process is essential for health.

Also, this process exists on a subcellular level – autophagy. Accordingly, it is the maintenance service in our cells, metaphorically speaking.

Instead of replacing the whole device (apoptosis), only the defective part is replaced (autophagy).

Thus, autophagy destroys subcellular organelles and builds new ones to replace them. The dysfunctional cell parts are sent to lysosomes as part of the process. And these lysosomes are unique organelles that contain enzymes to break down proteins.

In this sense, autophagy has three essential tasks in our cells:

  • Remove dysfunctional proteins and organelles
  • Remove pathogens
  • Prevent atypical protein accumulation
Autophagy protects the brain against neurodegeneration

Nobel Prize for Autophagy

Even Two Nobel Prize winners accompanied the term autophagy.

Since he discovered that lysosomes could break down cell components, the Belgian biochemist Christian de Duve was the first to shape the term autophagy.

Furthermore, he won the Nobel Prize in 1974 for this fundamental discovery.

However, a Japanese scientist started decisive experiments on yeast cells in the early 1990s.

Lastly, it was Yoshinori Ohsumi that showed precisely how the processes in autophagy work and how crucial they are for health.

Therefore, he was awarded the Nobel Prize in Medicine in 2016 for discovering the mechanism induced by fasting.

Moreover, Yoshinori Ohsumi called his Nobel Lecture: “Autophagy – an intracellular recycling system” (Levine et al. 20171).

Autophagy and Intermittent Fasting

However, the advantages of fasting are not limited to autophagy. Because, in principle, fasting induces two substantial mechanisms:

  • Activation of autophagy: Disposal of proteins and cell parts
  • Activation of growth hormone: Production of new, more powerful parts

Therefore, the combination of these mechanisms of fasting represents a complete renovation of the household in which we live.

Thus, fasting can reverse the entire aging process, as the body screens for dysfunctional cell parts and replaces them with new ones.

As we can often witness in nature, autophagy also has a certain balance. Because even from the best things, one can sometimes get too much.

Therefore both too much and too little autophagy can harm health. In times of abundance, we humans have forgotten a healthy balance between eating and fasting.

And intermittent fasting is the simplest way to restore this balance. Thus, intermittent fasting enables cell growth during eating and cleansing during fasting.

Accordingly, you can even use this simple principle to build muscle sustainably.

Furthermore, research has shown that even short fasting periods activate neuronal autophagy and thus protect the brain from neurodegenerative diseases such as Alzheimer’s or Parkinson’s (Alirezaei et al. 20102).

When Does Autophagy Start?

Accordingly, nature has provided a certain balance for humankind:

  • On the one hand, we grow in times of abundant food
  • On the other hand, we can live on body fat for weeks or months during food scarcity (Stewart et al. 19733)
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When food is scarce, the body does not want to grow. Thus, it’s a nutrient deprivation that induces autophagy.

Therefore, the human body has nutrient sensors that shut down or induce autophagy. Accordingly, there are three primary nutrient sensors:

  • Insulin: Sensitive to carbohydrates and proteins
  • mTOR: Sensitive to proteins
  • AMPK: Sensitive to lack of energy in cells

As soon as nutrient sensors detect low nutrient availability, autophagy kicks in.

Accordingly, the storage hormone insulin decreases during fasting, and its opponent glucagon, which induces consumption of stored energy, increases.

As a result, the increase in glucagon activates autophagy. In short, increasing glucagon by fasting is the most potent activator of autophagy.

Furthermore, mTOR (mechanistic or mammalian target of rapamycin) regulates autophagy. And this enzyme is crucial for growth.

Therefore, mTOR is extremely sensitive to proteins. When mTOR is low, it induces autophagy. However, when you stimulate mTOR, autophagy stops.

And the last primary activator of autophagy is AMP-activated protein kinase (AMPK). This enzyme indicates the energy stored in a cell. However, high AMPK means a low energy level, and low AMPK represents a high energy level.

So if the cell’s battery is empty, AMPK increases and activates autophagy.

When cells have no energy, they want to act sustainably and recycle old proteins to produce energy.

Autophagy kicks in as long as you do not stimulate any of these three nutrient sensors for about 14 hours (Yang et al. 20174).

Hence, even 16/8 intermittent fasting induces autophagy. Moreover, you can induce autophagy faster through exercise and nutrition.

What Breaks Autophagy?

Autophagy stops when we eat.

Since mTOR is the most central nutrient sensor, it integrates the signals from insulin and AMPK to determine whether the cell should divide and grow or go into a resting state.

As AMPK responds to all forms of energy, any macronutrient can activate both mTOR and AMPK:

  • Carbohydrates
  • Proteins
  • Fat

Therefore, even a tiny amount of glucose or amino acids can break autophagy.

With this in mind, autophagy occurs only when fasting. Simple calorie restrictions or diets cannot activate autophagy.

When the nutrient sensors are activated, we signal our body that we want to grow. Thus, nutrient sensors stop autophagy.

For this reason, autophagy is a catabolic (degrading) and not an anabolic (building) mechanism. However, there is a low basal level of autophagy at any given time because it is our bodies’ maintenance service.

Hence, excess nutrients put the body into growth mode via mTOR. On the other hand, fasting – the absence of nutrients – puts your body in maintenance mode.

How to Induce Autophagy

There are several scientifically proven methods to activate autophagy or to accelerate the achievement of autophagy induced by fasting:

1. Fasting

As we know, fasting is the primary tool to induce autophagy.

If you do not directly or indirectly activate the central nutrient sensor mTOR for at least 14 hours, autophagy kicks in (Yang et al. 20175).

According to animal studies, fasting can induce neural autophagy, particularly useful in preventing neurodegenerative diseases such as dementia (Alirezaei et al. 20106).

For this reason, many people report a sharpened focus and mental clarity when fasting.

In this sense, skipping breakfast is the easiest way to start fasting.

If you eat twice a day (i.e., at noon and 8 PM) and stick to unsweetened tea, water, or coffee without milk during fasting, you have already established a 16/8 intermittent fasting schedule.

Since morning hunger is a learned habit, you will quickly unlearn it and begin to benefit from autophagy.

woman fasting to induce autophagy

2. Keto Diet

If you are keto- or fat-adapted, fasting can help you reach a significant state of autophagy more quickly.

Since keto, like fasting, is based on lowering insulin, emptying carbohydrate stores, and burning fat as a primary fuel, you are already on the road to autophagy.

Additionally, a ketogenic diet also activates the AMPK signaling pathway, another primary sensor inducing autophagy (Paoli et al. 20157).

With this in mind, a keto diet in rats induced autophagy and protected their brains from possible seizures (Wang et al. 20188).

3. Regular Exercise

In motion-related autophagy, lifelong exercise induces the most remarkable effects.

Accordingly, one study shows that people who have played soccer have far more autophagy markers based on gene activity than people of the same age who have not exercised regularly throughout their lives (Mancini et al. 20199).

Also, scientists could detect autophagy in the liver, muscles, pancreas, and adipose tissue in mice regularly trained on the treadmill (He et al. 201210).

4. Intense Exercise

According to studies, exercise intensity is more decisive for muscle autophagy than a fasted state (Schwalm et al. 201511).

Accordingly, HIIT (High-Intensity Interval Training) is probably the best way to induce autophagy quickly.

Foods That Induce Autophagy

Furthermore, certain nutrients in food can induce autophagy:

1. Coffee

Both caffeinated and decaffeinated coffee activates autophagy in muscle tissue, the liver, the heart, and other vital organs – at least in mice (Pietrocola et al. 201412).

When they were fed, this effect occurred as well. Hence, the mice did not have to quickly induce autophagy through coffee.

Since decaffeinated coffee also works, it is probably the antioxidants in coffee that account for these benefits of autophagy.

coffee helps to induce autophagy

2. Red Wine

Resveratrol, a polyphenol, can also induce autophagy (Park et al. 201613).

Polyphenols are bioactive, antioxidant components known to be contained in red wine.

Although it is questionable whether you should drink more significant amounts of red wine to induce autophagy due to its alcohol content, one glass per day isn’t harmful.

Accordingly, scientists say this amount promotes health benefits (Yuan et al. 199714).

3. Green Tea

Just like red wine, green tea is full of bioactive polyphenols.

Accordingly, green tea consists of about 4000 different active compounds, but the most vital health benefits come from a particular class of polyphenols called catechins.

Accordingly, a recent study confirmed that the polyphenols in green tea induce autophagy (Prasanth et al. 201915).

Furthermore, green tea’s primary catechin epigallocatechin gallate (EGCG) can combat insulin-induced aging effects, type 2 diabetes, fatty liver, and lung cancer (Thielecke et al. 200916).

Due to its degree of fermentation, green tea outperforms black tea as it contains more polyphenols. Thus, green tea is a better choice than black tea, concerning autophagy and general mortality (Kuriyama et al. 200617).

4. Olive Oil

A recent study suggests that the anti-cancer properties of the most crucial antioxidant in extra virgin olive oil, oleuropein, occur by inducing autophagy (Przychodzen et al. 201918).

Hence, this represents further scientific evidence of the positive effect of the Mediterranean diet on life expectancy, of which olive oil is the cornerstone.

For example, in the southern Italian village of Acciaroli, more than 300 people are over 100 years old and have virtually no age-related diseases such as dementia or strokes.

5. Turmeric

Curcumin is the primary phytonutrient in turmeric.

It is instrumental in activating mitophagy – autophagy in our cellular power plants, mitochondria (de Oliveira et al. 201619).

Therefore, if you use turmeric as a seasoning for your favorite dishes, always combine it with black pepper. Why?

Since pepper contains piperine, it can increase the autophagy-inducing effects of curcumin by a factor of 20 (Shoba et al. 199820).

If you want to use a dietary supplement instead, although organic turmeric powder is a more sustainable way, it should also contain black pepper.

turmeric helps to start autophagy

6. Berberine

Berberine is a bioactive ingredient that occurs naturally in barberry, tree turmeric, Oregon grape, goldenseal, or yellowroot.

According to studies, berberine can activate autophagy (Jin et al. 201721).

You will most likely find berberine in food supplements, such as powder or pills.

Autophagy Fasting Benefits

As we have already experienced, too much growth and protein accumulation harm health.

Thus, modern diseases are all characterized by excessive growth – from atherosclerosis over cancer to polycystic ovary syndrome.

All these diseases are treatable through dietary intervention, especially fasting, which fights causes instead of symptoms, unlike drugs.

Furthermore, fasting-induced autophagy can repair age-related defects in cells. Therefore, the following vital effects are among the health benefits of autophagy:

1. Brain, Alzheimer’s, and Parkinson’s

Autophagy can help brain cells to excrete toxic proteins that contribute to dementia.

As they stick to neurons in the brain, the accumulation of these toxic proteins (β-Amyloid and Tau) significantly contributes to Alzheimer’s, Parkinson’s, and Huntington’s disease.

Notably, autophagy in the brain decreases significantly in the early stages of dementia (Li et al. 201722).

In contrast, fasting induces autophagy in the brain. Thus, fasting can protect against neurodegenerative diseases such as Alzheimer’s and Parkinson’s (Raefsky et al. 201723).

2. Aging and Longevity

Both fasting and resveratrol induced autophagy and thus increased life expectancy in animals.

As soon as the scientists removed autophagy-stimulating genes in these animals, the positive effect on life expectancy by fasting or resveratrol also disappeared (Nakamura et al. 201824).

Hence, scientists derive that autophagy is the leading cause that slows the aging process.

3. Cancer

On the one hand, autophagy repairs precisely those damaged proteins that contribute to tumor formation –known as the tumor suppressor effect (Yang et al. 201725).On the other hand, dysfunctional autophagy can also contribute to cancer.

Since tumors can be suppressed or promoted depending on their stage of development and tumor type, research intensively investigates the modulation of autophagy for cancer treatment.

With this in mind, researchers were able to use EGCG –  the primary polyphenol in green tea –  in a new treatment method to induce autophagy that triggered apoptosis, the death of the cancer cells (Prasanth et al. 201926).

Furthermore, studies suggest that influencing autophagy through intermittent fasting may improve cancer therapies’ effectiveness while protecting healthy cells (Antunes et al. 201827).

4. Insulin Resistance and Diabetes

autophagy fasting helps to reduce diabetes risk

Autophagy protects the insulin-producing beta cells in the pancreas (Yang et al. 201728).

When autophagy stops working correctly, the beta cells of the pancreas become damaged and eventually stop producing insulin.

Therefore, the adverse impairment of autophagy is a possible trigger of type 2 diabetes. Thus, increased autophagy can already be a preventive measure against pre-diabetes.

Furthermore, intermittent fasting can reverse the pre-existing disease known as insulin resistance and type 2 diabetes, which diets alone hardly can (Halberg et al. 200529).

5. Muscle Atrophy

Scientists have found that autophagy maintains and protects muscle tissue.

Also, autophagy can prevent age-related muscle atrophy. This effect on skeletal muscles indicates that autophagy can slow down the systematic aging process (Jiao et al. 201730).

6. Osteoporosis

Autophagy can counteract not only muscle atrophy but also bone loss.

Thus, studies suggest that autophagy also does the housekeeping in bone cells and thus recycles dysfunctional bone cells (Florencio-Silva et al. 201731).

7. Heart Health

Autophagy does not stop at the heart either. In this sense, research shows more and more that autophagy can probably renew damaged proteins and organelles in heart cells.

Thus, researchers conclude that autophagy can significantly reduce cardiovascular diseases (Sasaki et al. 201732).

intermittent fasting for beginners book

Consistency Will Induce Autophagy

The health benefits of autophagy open up an entirely new therapeutic potential.

If we do not activate our nutrient sensors for at least 14 hours, we can stop much of the growth that is now making us sick.

Since both carbohydrates, fat, and protein activate nutrient sensors, these are the crucial rules for autophagy fasting:

  • No eating, no snacking
  • Only water, tea, and coffee
  • No milk or milk substitutes
  • No sugar or sweeteners

With this in mind, even non-caloric sweeteners such as aspartame or stevia stimulate the nutrient sensor insulin (Anton et al. 201033).

Consistent fasting is the most effective way to prevent modern diseases of excessive growth, such as obesity, diabetes, PCOS, and cancer.

Learn how to successfully integrate fasting into your daily routine in my new book: Intermittent Fasting 101: The Science-Backed Beginner’s Guide to Lose Weight Without Dieting and Working Out.

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Induce Autophagy FAQ

How long does it take for autophagy to start?

According to studies, autophagy kicks in after about 14 hours. As you will learn in this article, exercise, a ketogenic diet, and certain foods can speed up the process.

Does fat stop autophagy?

Yes, all macronutrients, such as fat, will stop autophagy.

Does coffee affect autophagy?

Coffee without sugar, sweetener, milk or any milk substitute can even increase autophagy. Mainly religious reasons prohibit coffee during fasting. Learn more in my guide to coffee on intermittent fasting.

Does exercise induce autophagy?

Research has shown that regular and particularly intensive exercise can induce autophagy.

Studies

#1-7

1Levine B, Klionsky DJ. Autophagy wins the 2016 Nobel Prize in Physiology or Medicine: Breakthroughs in baker’s yeast fuel advances in biomedical research. Proc Natl Acad Sci U S A. 2017 Jan 10;114(2):201-205. doi: 10.1073/pnas.1619876114. Epub 2016 Dec 30. PubMed PMID: 28039434; PubMed Central PMCID: PMC5240711.

2Alirezaei M, Kemball CC, Flynn CT, Wood MR, Whitton JL, Kiosses WB. Short-term fasting induces profound neuronal autophagy. Autophagy. 2010 Aug;6(6):702-10. doi: 10.4161/auto.6.6.12376. Epub 2010 Aug 14. PubMed PMID: 20534972; PubMed Central PMCID: PMC3106288.

3Stewart WK, Fleming LW. Features of a successful therapeutic fast of 382 days’ duration. Postgrad Med J. 1973 Mar;49(569):203-9. doi: 10.1136/pgmj.49.569.203. PubMed PMID: 4803438; PubMed Central PMCID: PMC2495396.

4Yang JS, Lu CC, Kuo SC, Hsu YM, Tsai SC, Chen SY, Chen YT, Lin YJ, Huang YC, Chen CJ, Lin WD, Liao WL, Lin WY, Liu YH, Sheu JC, Tsai FJ. Autophagy and its link to type II diabetes mellitus. Biomedicine (Taipei). 2017 Jun;7(2):8. doi: 10.1051/bmdcn/2017070201. Epub 2017 Jun 14. PubMed PMID: 28612706; PubMed Central PMCID: PMC5479440.

5Yang JS, Lu CC, Kuo SC, Hsu YM, Tsai SC, Chen SY, Chen YT, Lin YJ, Huang YC, Chen CJ, Lin WD, Liao WL, Lin WY, Liu YH, Sheu JC, Tsai FJ. Autophagy and its link to type II diabetes mellitus. Biomedicine (Taipei). 2017 Jun;7(2):8. doi: 10.1051/bmdcn/2017070201. Epub 2017 Jun 14. PubMed PMID: 28612706; PubMed Central PMCID: PMC5479440.

6Alirezaei M, Kemball CC, Flynn CT, Wood MR, Whitton JL, Kiosses WB. Short-term fasting induces profound neuronal autophagy. Autophagy. 2010 Aug;6(6):702-10. doi: 10.4161/auto.6.6.12376. Epub 2010 Aug 14. PubMed PMID: 20534972; PubMed Central PMCID: PMC3106288.

7Paoli A, Bosco G, Camporesi EM, Mangar D. Ketosis, ketogenic diet and food intake control: a complex relationship. Front Psychol. 2015;6:27. doi: 10.3389/fpsyg.2015.00027. eCollection 2015. Review. PubMed PMID: 25698989; PubMed Central PMCID: PMC4313585.

#8-13

8Wang BH, Hou Q, Lu YQ, Jia MM, Qiu T, Wang XH, Zhang ZX, Jiang Y. Ketogenic diet attenuates neuronal injury via autophagy and mitochondrial pathways in pentylenetetrazol-kindled seizures. Brain Res. 2018 Jan 1;1678:106-115. doi: 10.1016/j.brainres.2017.10.009. Epub 2017 Oct 19. PubMed PMID: 29056525.

9Mancini A, Vitucci D, Randers MB, Schmidt JF, Hagman M, Andersen TR, Imperlini E, Mandola A, Orrù S, Krustrup P, Buono P. Lifelong Football Training: Effects on Autophagy and Healthy Longevity Promotion. Front Physiol. 2019;10:132. doi: 10.3389/fphys.2019.00132. eCollection 2019. PubMed PMID: 30837897; PubMed Central PMCID: PMC6390296.

10He C, Sumpter R Jr, Levine B. Exercise induces autophagy in peripheral tissues and in the brain. Autophagy. 2012 Oct;8(10):1548-51. doi: 10.4161/auto.21327. Epub 2012 Aug 15. PubMed PMID: 22892563; PubMed Central PMCID: PMC3463459.

11Schwalm C, Jamart C, Benoit N, Naslain D, Prémont C, Prévet J, Van Thienen R, Deldicque L, Francaux M. Activation of autophagy in human skeletal muscle is dependent on exercise intensity and AMPK activation. FASEB J. 2015 Aug;29(8):3515-26. doi: 10.1096/fj.14-267187. Epub 2015 May 8. PubMed PMID: 25957282.

12Pietrocola F, Malik SA, Mariño G, Vacchelli E, Senovilla L, Chaba K, Niso-Santano M, Maiuri MC, Madeo F, Kroemer G. Coffee induces autophagy in vivo. Cell Cycle. 2014;13(12):1987-94. doi: 10.4161/cc.28929. Epub 2014 Apr 25. PubMed PMID: 24769862; PubMed Central PMCID: PMC4111762.

13Park D, Jeong H, Lee MN, Koh A, Kwon O, Yang YR, Noh J, Suh PG, Park H, Ryu SH. Resveratrol induces autophagy by directly inhibiting mTOR through ATP competition. Sci Rep. 2016 Feb 23;6:21772. doi: 10.1038/srep21772. PubMed PMID: 26902888; PubMed Central PMCID: PMC4763238.

#14-19

14Yuan JM, Ross RK, Gao YT, Henderson BE, Yu MC. Follow up study of moderate alcohol intake and mortality among middle aged men in Shanghai, China. BMJ. 1997 Jan 4;314(7073):18-23. doi: 10.1136/bmj.314.7073.18. PubMed PMID: 9001474; PubMed Central PMCID: PMC2125578.

15Prasanth MI, Sivamaruthi BS, Chaiyasut C, Tencomnao T. A Review of the Role of Green Tea (Camellia sinensis) in Antiphotoaging, Stress Resistance, Neuroprotection, and Autophagy. Nutrients. 2019 Feb 23;11(2). doi: 10.3390/nu11020474. Review. PubMed PMID: 30813433; PubMed Central PMCID: PMC6412948.

16Thielecke F, Boschmann M. The potential role of green tea catechins in the prevention of the metabolic syndrome – a review. Phytochemistry. 2009 Jan;70(1):11-24. doi: 10.1016/j.phytochem.2008.11.011. Epub 2009 Jan 13. Review. PubMed PMID: 19147161.

17Kuriyama S, Shimazu T, Ohmori K, Kikuchi N, Nakaya N, Nishino Y, Tsubono Y, Tsuji I. Green tea consumption and mortality due to cardiovascular disease, cancer, and all causes in Japan: the Ohsaki study. JAMA. 2006 Sep 13;296(10):1255-65. doi: 10.1001/jama.296.10.1255. PubMed PMID: 16968850.

18Przychodzen P, Wyszkowska R, Gorzynik-Debicka M, Kostrzewa T, Kuban-Jankowska A, Gorska-Ponikowska M. Anticancer Potential of Oleuropein, the Polyphenol of Olive Oil, With 2-Methoxyestradiol, Separately or in Combination, in Human Osteosarcoma Cells. Anticancer Res. 2019 Mar;39(3):1243-1251. doi: 10.21873/anticanres.13234. PubMed PMID: 30842154.

19de Oliveira MR, Jardim FR, Setzer WN, Nabavi SM, Nabavi SF. Curcumin, mitochondrial biogenesis, and mitophagy: Exploring recent data and indicating future needs. Biotechnol Adv. 2016 Sep-Oct;34(5):813-826. doi: 10.1016/j.biotechadv.2016.04.004. Epub 2016 May 1. Review. PubMed PMID: 27143655.

#20-26

20Shoba G, Joy D, Joseph T, Majeed M, Rajendran R, Srinivas PS. Influence of piperine on the pharmacokinetics of curcumin in animals and human volunteers. Planta Med. 1998 May;64(4):353-6. doi: 10.1055/s-2006-957450. PubMed PMID: 9619120.

21Jin Y, Liu S, Ma Q, Xiao D, Chen L. Berberine enhances the AMPK activation and autophagy and mitigates high glucose-induced apoptosis of mouse podocytes. Eur J Pharmacol. 2017 Jan 5;794:106-114. doi: 10.1016/j.ejphar.2016.11.037. Epub 2016 Nov 22. PubMed PMID: 27887947.

22Li X, Chen H, Guan Y, Li X, Lei L, Liu J, Yin L, Liu G, Wang Z. Acetic acid activates the AMP-activated protein kinase signaling pathway to regulate lipid metabolism in bovine hepatocytes. PLoS One. 2013;8(7):e67880. doi: 10.1371/journal.pone.0067880. Print 2013. PubMed PMID: 23861826; PubMed Central PMCID: PMC3701595.

23Raefsky SM, Mattson MP. Adaptive responses of neuronal mitochondria to bioenergetic challenges: Roles in neuroplasticity and disease resistance. Free Radic Biol Med. 2017 Jan;102:203-216. doi: 10.1016/j.freeradbiomed.2016.11.045. Epub 2016 Nov 29. Review. PubMed PMID: 27908782; PubMed Central PMCID: PMC5209274.

24Nakamura S, Yoshimori T. Autophagy and Longevity. Mol Cells. 2018 Jan 31;41(1):65-72. doi: 10.14348/molcells.2018.2333. Epub 2018 Jan 23. Review. PubMed PMID: 29370695; PubMed Central PMCID: PMC5792715.

25Yang JS, Lu CC, Kuo SC, Hsu YM, Tsai SC, Chen SY, Chen YT, Lin YJ, Huang YC, Chen CJ, Lin WD, Liao WL, Lin WY, Liu YH, Sheu JC, Tsai FJ. Autophagy and its link to type II diabetes mellitus. Biomedicine (Taipei). 2017 Jun;7(2):8. doi: 10.1051/bmdcn/2017070201. Epub 2017 Jun 14. PubMed PMID: 28612706; PubMed Central PMCID: PMC5479440.

26Prasanth MI, Sivamaruthi BS, Chaiyasut C, Tencomnao T. A Review of the Role of Green Tea (Camellia sinensis) in Antiphotoaging, Stress Resistance, Neuroprotection, and Autophagy. Nutrients. 2019 Feb 23;11(2). doi: 10.3390/nu11020474. Review. PubMed PMID: 30813433; PubMed Central PMCID: PMC6412948.

#27-33

27Antunes F, Erustes AG, Costa AJ, Nascimento AC, Bincoletto C, Ureshino RP, Pereira GJS, Smaili SS. Autophagy and intermittent fasting: the connection for cancer therapy?. Clinics (Sao Paulo). 2018 Dec 10;73(suppl 1):e814s. doi: 10.6061/clinics/2018/e814s. Review. PubMed PMID: 30540126; PubMed Central PMCID: PMC6257056.

28Yang JS, Lu CC, Kuo SC, Hsu YM, Tsai SC, Chen SY, Chen YT, Lin YJ, Huang YC, Chen CJ, Lin WD, Liao WL, Lin WY, Liu YH, Sheu JC, Tsai FJ. Autophagy and its link to type II diabetes mellitus. Biomedicine (Taipei). 2017 Jun;7(2):8. doi: 10.1051/bmdcn/2017070201. Epub 2017 Jun 14. PubMed PMID: 28612706; PubMed Central PMCID: PMC5479440.

29Halberg N, Henriksen M, Söderhamn N, Stallknecht B, Ploug T, Schjerling P, Dela F. Effect of intermittent fasting and refeeding on insulin action in healthy men. J Appl Physiol (1985). 2005 Dec;99(6):2128-36. doi: 10.1152/japplphysiol.00683.2005. Epub 2005 Jul 28. PubMed PMID: 16051710.

30Jiao J, Demontis F. Skeletal muscle autophagy and its role in sarcopenia and organismal aging. Curr Opin Pharmacol. 2017 Jun;34:1-6. doi: 10.1016/j.coph.2017.03.009. Epub 2017 Apr 10. Review. PubMed PMID: 28407519.

31Florencio-Silva R, Sasso GR, Simões MJ, Simões RS, Baracat MC, Sasso-Cerri E, Cerri PS. Osteoporosis and autophagy: What is the relationship?. Rev Assoc Med Bras (1992). 2017 Feb;63(2):173-179. doi: 10.1590/1806-9282.63.02.173. PubMed PMID: 28355379.

32Sasaki Y, Ikeda Y, Iwabayashi M, Akasaki Y, Ohishi M. The Impact of Autophagy on Cardiovascular Senescence and Diseases. Int Heart J. 2017 Oct 21;58(5):666-673. doi: 10.1536/ihj.17-246. Epub 2017 Sep 30. Review. PubMed PMID: 28966332.

33Anton SD, Martin CK, Han H, Coulon S, Cefalu WT, Geiselman P, Williamson DA. Effects of stevia, aspartame, and sucrose on food intake, satiety, and postprandial glucose and insulin levels. Appetite. 2010 Aug;55(1):37-43. doi: 10.1016/j.appet.2010.03.009. Epub 2010 Mar 18. PubMed PMID: 20303371; PubMed Central PMCID: PMC2900484.

This Post Has 17 Comments

  1. Stephen

    Extremely well written and informative article. Takes alot of information, out on the net, and brings it together. Great job.

    1. Hi Stephen,

      Thanks for your time to submit feedback. I am delighted that the information was helpful, and you appreciated that the article is backed by more science-backed sources than the average one 🙂

      Have a great day and br,
      Stephan

    2. Esteri

      I just bought your book off of amazon to compliment the information from Dr. Fung. Thank you for your work. I am doing alternate day fasting and wondering if I need to be concerned about not meeting my DV% for nutrients on the fasting days. Can I make up for it on the eating days? So for example we need about 4000 mg of potassium a day but that would mean I have to hit 8000 on my eating day and double for all the other nutrients. Another question would taking 2.5 grams of camu powder on my fasting days throw off autophagy?

      1. Hi Esteri,

        I am delighted my work was helpful to you – thanks for the cheerful words!

        1) Yes, the human body can store nutrients – that’s why we do not need to eat round the clock, as the food industry has told us. Find this and other myths about fasting debunked by science here.
        2) Technically, Camu Powder breaks a fast since it’s 70% sugar. Your serving since is not much, but it will at least diminish autophagy – so it’s up to you to decide.

        Have a great day,
        Stephan

  2. Steve G.

    I am confused by this article.

    It says that Autophagy stops we when we eat, ergo:
    ~ Carbohydrates
    ~ Proteins
    ~ Fat

    and yet a Keto diet, which is high fat, one of the nutrients listed above, can induce Autophagy.

    So does eating fat stop Autophagy, or induce it… which is it?

    Thanks

    1. Hi Steve,

      Thanks for the question.

      As the article states: If you are keto-adapted, fasting can help you reach a significant state of autophagy more quickly.

      So if your regular diet is high-fat low-carb, your insulin levels are way lower (healthier), and you can reach autophagy with fasting or working out more quickly. In contrast, if you are on a Western Pattern Diet (WPD) high in carbs, you might need 30 hours to reach a significant state of autophagy with fasting or even more. For this reason, it is so hard to tell when autophagy starts individually since it depends on lifestyle factors.

      BR,
      Stephan

  3. Swami Saran Sharna

    Hi Steve,
    Thank you for the very well compiled and explained article.
    I have been reading on and off on autophagy and the related subjects but this is for the first time, I feel that I have got the gist.
    Thanks again and have a good day.
    Swami

  4. Jackie Harrell

    Hi, thank you for the article. One section confused me and I’d like a little clarification please. Olive oil is listed specifically as inducing Autophagy. Then later the article says fat stimulates the nutrient sensing pathways which then stop Autophagy. How can olive oil both stimulate Autophagy AND stop Autophagy? Is it dose related? Perhaps you could clarify the situation for me. Again, please and thank you.

    1. Hi Jackie,

      thank you for your kind words! 🙂

      The thing about food inducing autophagy is that it usually has macro-nutrients and hence stimulates one of the nutrient sensors in our bodies. Olive oil, however, is one of the best choices since it can only have an impact through AMPK and won’t stop ketosis. While there is no 100% on and off switch regarding autophagy, I suggest consuming autophagy-enhancing food in your diet, especially with meals before starting a fast.

      BR,
      Stephan

  5. Esteri

    Interesting you say there is no on and off switch for autophagy as that brings back to mind a comment I heard on a podcast that it is more like a dimmer switch getting weaker or stronger. So does that mean when I do a 36 hour fast to get into the best autophagy that if I need some fasting aids like bone broth or a bit of cream in my coffee I haven’t ruined my efforts to induce autophagy? If I needed a fasting aid just to extend my fast as long as possible rather than quitting earlier what would you suggest (other than the usual coffee, tea, sparkling water)

  6. Julia Shelton

    If I am understanding this correctly, I can’t put any kind of sweetener or creamer of any kind in my coffee or it will curtail autophagy? Is honey included in that? Is there anything I can put in my coffee that will not affect autophagy? Thank you for your consideration.

    1. Hi, Julia, that is correct.

      There is not enough research to say if erythritol inhibits autophagy, but most likely, since other sugar alcohols like xylitol can break a fast (*). Moreover, even non-nutritive sweeteners like stevia inhibit autophagy since they directly induce insulin production in the pancreas without the intense effect on blood sugar (*).

      Although I prefer it to sweeteners, honey is mostly sugar – so that’s going to ruin autophagy.

      What is sold as “creamer” is absolute junk (50%+ refined sugar) – I would not put these substances in my coffee. If you want to lose weight put some real cream into your coffee – it still affects autophagy but not ketosis.

  7. Tabish

    Thanks but could you please suggest the amount of coffee or green tea to be taken?

  8. Nathan

    Here’s a question. Is the 14 hour delay for autophagy to start absolute? If I’ve been fasting for 48 hours and then have some bone broth, sure, that will kick me out of my fast. But does it take another 14 hours before autophagy will start back up again or will it restart sooner since my glycogen stores are so depleted and the bone broth represented a minimal caloric load? Is it time based (a neural shot clock) or calorie based?

    1. Hi Nathan,

      If you only had bone broth, it would go faster 🙂

      Several ways can speed up the process, like exercise or a ketogenic diet (both deplete glycogen stores).

      Have a great day,
      Stephan

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